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Glutathione biosynthesis occurs in two sequential, ATP-dependent enzymatic steps that take place in the cytosol: Step 1: The enzyme glutamate-cysteine ligase (GCL) combines glutamate and cysteine to form gamma-glutamylcysteine

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[7] [2] By 1986, the major review of the field by Graf and Kastin concluded that the various physiological functions of DSIP and its mechanism of action remain to be established. [2] That sentence was written four decades ago and has not been superseded

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