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Pull-down assay and immunoblotting Interactions between exendin 939 and TRPV1 were examined via His-mediated pull-down assays using a modified protocol from the Pierce Pull-down PolyHis Protein:Protein Interaction Kit (Thermo Fisher Scientific)

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These observations are consistent with a crossover study in 20 adults reporting significantly higher fasting plasma TMAO in participants above median age (45.5 years) (62)