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Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution

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Withdrawal increased anxiety-like behaviors, possibly linked to the upregulation of the GLP-1 pathway

Changes in intestinal morphology, in particular villus hyperplasia, have been implicated in the adaptive response following Roux-en Y-Gastric bypass and ileal interposition in rats (118, 119)

If you have pre-existing medical conditions such as diabetes, kidney disease, or cardiovascular disease, or if you are taking other medications (such as diuretics, statins, or blood pressure medications) that may increase cramping risk, inform your healthcare provider