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For adherent SC-islets, SC-islets were first dissociated with 0.25% trypsin and seeded onto 33-mm confocal dishes (Beyotime, #FCFC020-150pcs) coated with Matrigel diluted to 1:3

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Prolonged use of topical niacinamide might be accompanied by some mild adverse reactions including mild burning, erythema, and pruritus (Taylor et al., 2003)

2A), except for artifacts