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Classification of DAMPs Protein-based DAMPs HMGB-1 HMGB1, among the most comprehensively characterized DAMPs, is a 215-amino acid non-histone nuclear protein that consists of three structural domains: two tandem high-mobility group (HMG) box domains (Box A and Box B) and a 30-amino acid-rich C-terminal tail [44]

2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

Numerous studies have suggested that NADPH oxidase and iNOS can generate synergistic effects in inducing cell death [152, 153]

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