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6a, b, HCT116 cells treated with BMS-754807 and U0126 induced p70S6K1 phosphorylation (lanes 2 and 3)

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to activate vagal afferents and measured the density of Fos immunostained nuclei in the NTS and PVH of GCG-Cre::SynTom::Lep ob/ob mice and WT controls