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One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

Both of which can lessen the appearance of dark circles

Reconstitution Quick Reference Because AHK-Cu is prepared as a topical serum, "reconstitution" here means dissolving the dry powder into a carrier to reach a target concentration, not drawing units for injection

Avoid taking it close to bedtime as NAD elevation may impact circadian signaling

Conversely, a decrease in the bioavailability of 5-methylTHF generates a reduction of homocysteine remethylation (Fig