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Deferoxamine (DFO), an FDA-approved iron chelator, effectively inhibits ferroptosis by binding free Fe 3 , decreasing ROS production, and upregulating ferroptosis-protective proteins such as GPX4, ferritin heavy chain 1 (FTH1), and system x [30]

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Glutathione synthesis and turnover in the human erythrocyte

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These measurements allow the calculation of the ratio of GSH/GSSG, or the ratio of reduced to oxidized glutathione, in order to determine the oxidative stress present in cultured mammalian cells in response to a compound