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Crosslinking mass spectrometry (XL-MS) The final concentration of 0.5 M DDD-Ube2e2-COP1-c-Jun was incubated with a final concentration of 0.1 mM DSBU crosslinker (Thermo Fisher, A35459) in the wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP) for 40 min at 25 C and quenched with 10 mM Tris-HCl pH 8.0 for 10 min at 25 C

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What Is In It Akkermansia muciniphila MucT (30 Billion AFU) as a pasteurized postbiotic, Black Ginger Root Extract (Kaempferia parviflora, 150mg), and Chromium Picolinate (200mcg, 571% Daily Value)

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