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Contact mode scratching of the substrates was performed to assess whether the monolayers had the expected protein heights, using a cantilever with a bare tip and a nominal spring constant of 0.5 N/m, by applying a force ranging from a few nN for imaging to hundreds of nN for scratching as reported in Funari et al

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Other symptoms include pelvic pressure or pain, frequent urination, difficulty emptying the bladder, and constipation