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Abcam, Cambridge, MA, USA), LC3 rabbit antibody (12741S, 1:1,000) and Beclin1 rabbit antibody (3495S, 1:1,000) (both from Cell Signaling Technology, Danvers, MA, USA) for 12 h at 4C, or with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (G8795, 1:1,000

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Biomarkers That Shape Dose Efficacy at the 5mg Level Beyond genetics, baseline biomarkersfasting insulin, HbA1c, liver enzymes, renal function, GLP-1 baseline levelsprovide clinical insight into whether 5mg is the right starting point

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This was motivated by a meta-analysis, which looked at 44 randomized controlled trials using Berberine, and found it may help protect against cardiovascular disease

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