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(5) Protein pellets were resuspended and vortexed in 250 L fresh RIPA buffer (150 mM NaCl, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) with 0.1 mM PMSF, following which they were incubated at room temp for 5 min

KR: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Supervision, Writing original draft, Writing review & editing

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Furthermore, in vitro experiments using cultured human retinal Muller glia and retinal pigment epithelial (RPE) cells showed that GSNO treatment reduced SA-induced inflammatory mediators and cell death

This helps reduce the cognitive and physical exhaustion associated with processing visual information, a common issue for those managing brain fog