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In patients with pre-existing heart failure, autophagy inhibition may exacerbate proteotoxic stress rather than confer benefit, prompting cautious evaluation in cardiac clinical trials

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Mass spectrometry Protein containing polyacrylamide gel slices were prepared for mass spectrometric analysis by 2 5 alternating washing steps with 10 mM HEPES, p H 6.8 in water and a 1:1 mixture (v/v) of 100 mM HEPES in water (p H 6.8) and acetonitrile