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tuberculosis strains N0155 (lineage 2) and N1283 (lineage 4) 65 were grown in Middlebrook 7H9 broth (BD Difco) with 10% (v/v) oleic albumin dextrose catalase, 0.2% (v/v) glycerol, 0.05% tyloxapol (w/v) and 0.1 mM sodium propionate (ThermoFisher) to optical density at 600 nm (OD 600 ) = 0.6 (ref