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Because of the difficulty of identifying specific sinusoidal cell types from H&E-stained sections, definitive diagnosis of endothelial cell hypertrophy/karyomegaly requires confirmation using special stains
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These were allowed to adhere overnight in full culture media (DMEM (41965039) with 4 mM L-glutamine, 10% dFBS (26400044), 1 mM sodium pyruvate (11360039) and 50 U ml 1 penicillinstreptomycin (15070063)) under normal cell culture conditions
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