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Trials 1 and 2 observed a mean reduction in AHI of 25.3 and 29.3 events per hour with tirzepatide compared to 5.3 and 5.5 events per hour with placebo, respectively, and an average overall body weight reduction of up to 20% from baseline in the tirzepatide groups [127]

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To make sure all read values were in the range of the calibration curve, series dilutions were made from the samples

RNA quantity and quality were evaluated by Nanodrop 8000 (Thermo Fisher Scientific, Waltham, MA, USA) and Fragment Analyzer (Advanced Analytical Technologies, Heidelberg, Germany) using the DNF-471 Standard Sensitivity RNA Analysis Kit (15nt) (Agilent Technologies, Santa Clara, CA, USA)

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