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Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h

Promotes blood vessel growth

Their safety is unknown

Pharm Res 32:35263540 Jeger S, Zimmermann K, Blanc A, Grnberg J, Honer M, Hunziker P, Struthers H, Schibli R (2010) Site-specific and stoichiometric modification of antibodies by bacterial transglutaminase

Direct inhibition of copper accumulation with the chelator BCS rescued the cold-tolerance defect of the slcr-46.1 mutant, whereas inhibition of the ferroptosis pathway did not