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R NAT2 polymorphisms alter catalytic activity primarily through effects on protein stability and degradation rates rather than changes to the active site itself (slow acetylator alleles produce unstable protein that is rapidly ubiquitinated and degraded)

The absence of needles was a significant advantage, although the need for consistent daily adherence was noted

Subsequently, the optical density at 570 nm was determined using a microplate reader

In order to minimize proteolysis, all steps of the protein purification should be carried out at 4 C, unless otherwise noted