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Total mtDNA copy number was determined using quantitative real-time PCR

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reported that TNF--antagonism was linked to a reduction in myocardial inflammation and fibrosis and that it improved diabetic cardiomyopathy that was experimentally induced [18]

The specific mechanisms by which ferroptosis contributes to CHD pathophysiology require further elucidation, particularly regarding its interrelationship with other cell death pathways (e.g., apoptosis, necrosis)

Cells were grown in LB medium with ampicillin, induced with 0.5 mM IPTG at 25 C for 1012 h, and harvested after cooling to 4 C