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Research by Statzer et al

In the cortical layer of the ovarian sections, follicles in which the oocyte nucleus and nucleolus could be distinctly identified were classified as primordial, primary, secondary, antral, or atretic

Plasma TNF assay Blood samples were drawn from the dorsal vena cava, and plasma was obtained by centrifuging the blood at 8000 g for 15 min at 4 C