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The rigidity of calf muscles is also present where the muscles in the back of the lower leg get hardened and stiff, adding to more suffering while walking

The oligomer was then diluted 1/50 with a solution of plasma/PBS pH 7.4 (1:1) to afford a final concentration of 5 M and incubated at 37 C (4 L of the stock solution was diluted with 196 L of plasma/PBS, pH 7.4, 1:1)

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