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Research has shown that enhanced expression of either PINK1 or Parkin in rat hippocampal neurons results in more mitochondria but smaller in size and volume, indicating a tendency towards more fission

When pretreating the cells with N -ethylmaleimide (NEM, 20 mM), a trapping reagent of thiol species, for 0.5 h, the signals from the cytoplasmic regions are decreased in both green and yellow channels (Figure 7DF)

216 To overcome this, combination therapies that block multiple Hedgehog signaling components or integrate Hedgehog inhibitors with metabolic and immune-targeting agents are currently being explored

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