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Following incubating with 2 M fura-2 AM (DOJINDO, Kumamoto, Japan) for 30 min at 37 C, the cells were mounted in chamber and superfused at 1.3 ml min 1 at 30 C with HKRB containing 5.6 mM glucose

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Considering the substantial global prevalence of neurological disorders among PLWH, it is imperative to gain a deeper understanding of the pathobiology and identify potential therapeutic targets to develop new strategies for the prevention and treatment of NeuroHIV