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Endosomal binding CHO-SNAP-GLP-1R cells were labeled with 5 M BG-SS-biotin, a cleavable SNAP-Surface probe containing a disulfide bond between O 6 -benzylguanine and biotin moieties (a gift from New England Biolabs) plus streptavidin-terbium cryptate (Cisbio, 0.24 g ml 1 active moiety)

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To check this, we incubated starved the gsh1 -mutant cells in SD-media to which we added 0 M, 0.25 M or 2.5 M glutathione

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doi: 10.1038/labinvest.3780057 63 PatelVBBodigaSBasuRDasSKWangWWangZet al

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Nature 569:270274

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