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Correspondingly, WB, IHC and IF techniques could be used for analyzing hub targets expression and signaling pathways in mice

Quantitative analysis of IHC/IF signals was conducted using ImageJ (NIH) to calculate integrated density values from 10 random fields per sample, while confocal data quantification (C11-BODIPY oxidation ratios, RhoNox-1/DCFH-DA intensities) utilized NIS-Elements AR 5.02 software