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Despite the added advantages of the freeze-drying method, there are a few disadvantages associated with it such as membrane integrity may be lost, encapsulated substances might leak out from the vesicle and vesicle size might decrease [163]

Zargar M, Shameli K, Najafi GR, Farahani F: Plant mediated green biosynthesis of silver nanoparticles using Vitex negundo L

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Proc Natl Acad Sci USA 109:1199311998 Wu G, Fang Y-Z, Yang S, Lupton JR, Turner ND (2004) Glutathione metabolism and its implications for health

NNMT methylates nicotinamide (a form of vitamin B3) into N-methylnicotinamide, consuming NAD+ in the process