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Strikingly, substitution of a Cys for Sec lowers the enzymatic activity of GPx-1 by orders of magnitude (309), leading to the suggestion that a different mechanism involving a second resolving Cys is essential for the enzymatic activity of normally occurring nonselenium ( i.e ., Cys-containing) forms of GPxs, such as the GPxs in yeast strains that lack a Sec incorporation mechanism (359)

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