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In one program, a separate NNMT inhibitor reduced 1-methylnicotinamide levels and improved insulin sensitivity and glucose tolerance in DIO mice, with these effects absent in NNMT-knockout animals

Since B12 is found primarily in animal products, including meat, fish, dairy, and eggs, a substantial reduction in food intake can reduce B12 consumption below optimal levels, particularly for patients who were already borderline deficient before starting treatment or whose diets lean toward plant-based foods

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