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Avoid rubbing the injection site immediately afterward

For immunofluorescence (IF) staining, after deparaffinization and antigen retrieval, sections were incubated overnight at 4 C with a mouse anti-insulin antibody (1:200, GB15334, Servicebio), rabbit anti-PPAR antibody (1:50, ab23673, Abcam), or rabbit anti-UCP2 (1:200, DF8626, Affinity) antibody, followed by incubation with appropriate secondary antibodies (1:1000, 7074 S and 7076 S, CST)

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Its use in clinical practice has been replaced by newer insulin-sensitising agents (sodium glucose cotransporter-2 [SGLT-2] inhibitors and glucagon-like peptide 1 [GLP-1] receptor agonists), with more pronounced effects on weight loss and cardiovascular benefits