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Each sample was resuspended in 20 l of LC/MS grade water and 10 l per sample was injected into a 5500 QTRAP, a hybrid triple quadrupole/linear ion trap mass spectrometer, using a quantitative polar metabolomics profiling platform with selected reaction monitoring (SRM) that covers all major metabolic pathways

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The reduction reaction was catalysed by PDI at room temperature, and the resulting aggregation of reduced insulin B chains was measured at 620 nm

As a naturally occurring growth factor, IGF-1 plays crucial roles in muscle development, fat metabolism, and cellular repair throughout your body

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