Proteins were separated in one dimension on Bolt 4%12% Bis-tris Plus gels (Invitrogen Thermo Scientific) using a mini protean vertical gel electrophoresis mini-tank module (Invitrogen Thermo Scientific)
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Other findings have indicated increased oxidative damage to DNA and a reduction in the levels of various antioxidants, including thiols (-SH), catalase, uric acid, glutathione, glutathione peroxidase, and ferric-inducing antioxidants [7, 125, 129, 130]
no thyroid function tests were done
Most side effects peak within the first one to two weeks of a new dose, then gradually improve as your body acclimates