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2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C

Consultation with a doctor is advised before using this medicine during pregnancy or while breastfeeding

Keep vials in the back of the refrigerator, not the door

Nevertheless, most of these biomarkers are diagnostic, meaning they are elevated by the time the disease is clinically present, thus with scarce predictive capacity

This might have resulted in an insufficient return to the threshold, as in the present study