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10.1073/pnas.0806720106 78 LockieS

To test whether leptin alters the activity of postsynaptic neurons in the PVH that receive GLP-1 inputs, we used whole-cell patch-clamp electrophysiology to record miniature excitatory postsynaptic currents (mEPSCs) from PVH neurons that express GLP-1 receptors (GLP-1 R), visualized by crossing Glp1r-Cre mice with the Cre-dependent fluorescent reporter tdTomato in leptin-deficient mice (GLP-1 R-Cre::tdTom::Lep ob/ob mice)

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"In addition, your response is inadequate because you did not provide sufficient details about proposed changes to policies and procedures being implemented at your site to prevent similar violations in the future