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Purified 15 N FAT was buffer exchanged into thrombin cleavage buffer (50 mM Tris-Cl pH 8.0, 10 M CaCl 2 ) on a HiLoad 26/60 Superdex 75 pg gel filtration column

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They reported significant body weight reductions and improvements in several risk factors, including blood pressure, cholesterol, and BMI (body mass index) among participants