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Real-Time Quantitative Reverse Transcription PCR (qRTPCR) Analysis Total RNA from skin tissues was isolated using TRIzol reagent (Sigma, Milwaukee, WI, USA), and 1 g of total RNA was reverse-transcribed into cDNA

Peyers patches (Pps), but not mesenteric lymph nodes (mLNs) of 5xFAD presented a higher frequency of T H 17 cells relative to WT mice, while Pps of GF 5xFAD mice showed a negligible level (Supplementary Fig

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Chemosensitivity profiling of XDPOs For chemosensitivity profiling, XDPOs were plated into 96-well plates and then subjected to incrementally increasing concentrations of drugs

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