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To evaluate intestinal barrier integrity, rectal sections underwent immunofluorescence staining for ZO-1 using a rabbit primary antibody and an Alexa Fluor 488 secondary antibody (green), and the nuclei were counterstained with DAPI (blue)

2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C

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It's important to note, however, that not all Parkinson's patients may be suitable for glutathione treatment, and other treatment options may be necessary