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Superoxide reacts with NO to form peroxynitrite (ONOO ), a toxic compound that depletes NO and worsens endothelial dysfunction (Li H

10.1016/j.tox.2009.09.017 Toxicology 60 ShimizuS.AtsumiR.ItokawaK.IwasakiM.AokiT.OnoC.et al (2009)

The Board has declared compounded sterile drug products adulterated if they contain nonpharmaceutical grade drug components such as glutathione or methylcobalamin

Saline solution (0.9% sodium chloride) is sometimes used but has disadvantages

A review published in Cureus (2024) noted that while TB-500 shows promise for soft tissue regeneration, additional research is necessary before clinical translation can occur