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Subsequently, cells were centrifuged at 4 C, 4,000 g for 15 min, then resuspended in cold buffer A (20 mM HEPES-Na + , pH 8.0, 500 mM NaCl, 1 mM TCEP) and sonicated three times for 15 min each

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10.1016/0165-1110(90)90014-3 34 FolgerA.WangY

In some cases, researchers can trace the problem to oxidative stress

5.1 Preserving the GSH/GSSG Redox State Across Sample Matrices Preanalytical oxidation is a major source of error in GSH/GSSG analysis