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The ice crystal formation physically damages the peptide structure, and this damage does not reverse when the solution thaws

The detrimental effects of B12 deficiencysuch as hyperhomocysteinemia and impaired methylationare well-established and can negatively impact oocyte quality and embryo viability [19, 20], which is consistent with the lower pregnancy rates we observed at low B12 levels

doi.org/10.3389/fphar.2021.627533 Chang, C

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