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cAMP measurement cAMP levels in whole brain tissues were measured using a cAMP assay kit (Shanghai Boke Biotechnology Co., Ltd., Shanghai, China)

Normal thyroid function is regulated by the hypothalamic thyrotropin-releasing hormone (TRH) that stimulates the release of the thyroid-stimulating hormone (TSH) from the pituitary gland, which in turn drives synthesis and secretion of the thyroid hormones from the thyroid gland ( th week of gestation, the embryonic thyroid is in its final position in front of the trachea