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Acknowledgements The authors thank the AMD Annals Study Group for access to the anonymized dataset and the AMD Foundation for institutional support

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12 Fasting does not consistently activate AMPK or decrease acetyl-CoA levels in the rodent cerebral cortex In many tissues and brain regions during fasting, ACACA and the rate limiting enzyme of cholesterol synthesis 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) are phosphorylated by AMPK to inhibit lipid synthesis (Muraleedharan and Dasgupta, 2022)