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The characterization of the specific binding of [ 3 H]- N -acetylaspartylglutamate to rat brain membranes

Kalaras, John P

An acidic environment can destabilize peptide bonds if you apply them at the same time

Subtle dynamics allows the C-terminal of the ligand to access the ECD binding site, and the binding of the ligand triggers further dissociation between the ECD and TMD, allowing the N-terminal to enter the orthosteric pocket in the TMD and activate the receptor