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With an envelope of 50 ms featuring 1.0-ms ramps and occurring 100 ms before the startle stimulus, the acoustic prepulse and the gap carrier were both filtered noise (one-third octave bandpass, 48 dB/octave roll-off) centered at 9.3, 16, 20, and 24 kHz, each at an intensity of 60 dB SPL

Prepare the needle by removing the needle cap with your non-writing hand