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Additionally, one study indicated that the accumulation of autophagic substrates and impaired autophagic flux independently signify suppressed autophagy post-TBI (Sarkar et al., 2014)

Melatonin was dissolved in a minimum volume of absolute ethanol plus tap water to yield a dose of 10 mg/kg of body weight

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it's a sterile water solution with a pinch of 0.9% benzyl alcohol, acting as a bacteriostatic agent