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In brief, worms were first lysed in 200 mM DTT, 0.25% SDS, 20 mM HEPES, and 3% sucrose with rocking at 700 rpm for 7 min at 20 C and subsequently digested in 20 mg/ml Pronase (Merck Millipore, 537088) and 2 mg/ml Collagenase IV (Worthington, LS004186) with rocking at 1200 rpm for 5 min at 20 C