Its important to note that in vitro models suggested that glutathione amino acid precursors are responsible for increasing skin thickness, but further research is required to better understand how topical application has any role in promoting skin structure, skin appearance and collagen creation
The study indicated that supplementation of cell culture medium with 100 nM of sodium selenite led to 2-, 5-, and 40-fold increase of GPx activity in HT29, P31, and N-18 cells, respectively
Molecules (2023) 28:4392
On the fourth day, 1 mg ml of l-(4, 5-Dimethylthiazol-2-yl) 3, 5-diphenyIformazan reagent (MTT, Sigma- Aldrich) prepared in PBS was added to the cells and incubated for 4 h
(PubMed) Christensen B, Arbour L, Tran P, et al