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Discontinuation rates are higher than existing medications

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This is because the elevated glycine would disrupt the interactions between GcvR and GcvA and allow expression of the glycine biosensor to occur (reaction 4), whereas there is no elevated glycine in reaction 5 so this interaction would not be disrupted

10.1093/gerona/58.12.M1103 13 BikleD

For the electron flow assays, mitochondria were plated in MAS buffer with 10 mM pyruvate + 2 mM malate + 4 M FCCP followed by 20 M rotenone in port A, 100 mM succinate in port B, 40 M antimycin A in port C and 1 mM TMPD + 100 mM ascorbic acid in port D