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A CellProfiler pipeline was designed to detect and count live and dead cells, mitochondria and lysosomes from immunofluorescence images using automatic thresholding and segmentation methods (see All experiments were repeated at least 2-3 times with different inductions (hiPSC derived neurons)

However, the full effects typically manifest over 3-6 months, with maximum weight loss usually occurring at 72 weeks (approximately 16-17 months)

172 The challenges in developing these drugs include ensuring that they can effectively mimic the biological activity of large molecule GLP-1RAs while maintaining efficacy and selectivity

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