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This was a cell-system endpoint, not a clinical outcome

LTF-treated mice expressed normal behavioral activities

Singly-housing animals, which prevents inter-animal coprophagy, reduces beta diversity of the microbiome compared to stressful (chronic subordinate colony) co-housing conditions 72

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The removal of biotin from lysine residues in proteins in the diet and from the carboxylases in cells, through the action of biotinidase, and then the incorporation of the biotin into the carboxylases, through the action of holocarboxylase synthetase, is referred to as the biotin cycle