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Subsequently, the membranes were incubated at 4 C overnight with the following primary antibodies diluted in antibody dilution buffer (Genefist, GF1600-02), SIRT1 (Santa Cruz, sc-74465,1:300), NRF2 (Affinity, #AF0639,1:1000), GPX4 (Proteintech, 67763-1-Ig,1:2000), and GAPDH (Proteintech, 60004-1-Ig,1:20000)
doi:10.1038/nrn3111
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